Progress ofin vitrofactor VIII coagulant activity from 0 to 8 hours after reconstitution

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Progress of in vitro factor VIII coagulant activity from 0 to 8 hours after reconstitution

BACKGROUND Continuous infusion of factor VIII (FVIII) is a more cost-effective method for treating hemophilia A than intermittent bolus injection. However, there is currently no specific data in Korea about the progress of in vitro FVIII coagulant activity (FVIII:C) after reconstitution from its lyophilized form. METHODS Three commercial FVIII concentrate products (two recombinant FVIII and o...

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Factor Viii Coagulant Activity and Factor Viii-like Antigen: Independent Molecular Entities

Factor VIII coagulant activity (VIII(C)) and the von Willebrand's disease antigen (Factor VIII-like antigen, vW-Ag) are biologically linked, and it has been suggested that they reside on the same molecule. However, insolubilized human isoantibody to VIII(C) and rabbit antiserum containing antibodies to VIII(C) and vW-Ag differentially bind and remove these entities from plasma, thus physically ...

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Inactivation of factor VIII coagulant activity by two different types of human antibodies.

Human antibodies that inactivate factor VIII procoagulant activity (VIII:C) are heterogeneous in their kinetic properties. We report there here the properties of four type I and four type II antibodies classified according to Biggs et al. Type I antibodies have second-order inactivation kinetics and completely destroy VIII:C when present in high concentration; type II antibodies have more compl...

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Monoclonal antibodies to porcine factor VIII coagulant and their use in the isolation of active coagulant protein.

Partially purified preparations of porcine factor VIII:C were used to immunize mice and spleen cells from the immunized animals were fused to NS-1 mouse myeloma cells. The ability of hybrid culture fluids to bind factor VIII:C was detected with a radiolabelled, affinity-purified, human antihuman VIII:C inhibitor. Three cloned hybrid lines have been obtained that preferentially bind to VIII:C wh...

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Lower Factor VIII Coagulant Activity in Daughters of Subjects With Hemophilia A Compared to Other Obligate Carriers

Studies of factor-VIll-related activities were performed in 62 obligate carriers of hemophilia A. comparing 23 daughters of hemophilic men (paternal carriers) with 39 mothers of hemophilic Sons whose fathers were normal by history (maternal carriers). Nineteen of the maternal carriers were mothers of at least 2 hemophiliacs and 20 were mothers of one. but from families whose other close male me...

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ژورنال

عنوان ژورنال: Blood Research

سال: 2014

ISSN: 2287-979X,2288-0011

DOI: 10.5045/br.2014.49.4.265